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KMID : 1094719990040040273
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 4 p.273 ~ p.276
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Improved paclitaxel production in a two-phase suspension culture ofTaxus cuspidata using silicone cubes
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Kim Chang-Heon
Hong Seong-Hyun
Son Sung-Ho
Chung In-Sik
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Abstract
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Two-phase cultures ofTaxus cuspidata were performed using silicone cubes as a second phase in shake flasks for paclitaxel production. Among various taxanes, paclitaxel was selectively adsorbed on the silicon cubes. When silicone cubes were added to suspension culture ofTaxus cuspidata, paclitaxel production increased about 45 folds. The maximum paclitaxel production was 3.95 mg/L when 10% of silicone cubes were added to the culture at the 7th day from inoculation.
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KEYWORD
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paclitaxel, Taxus cuspidata, suspension culture, two-phase culture, silicone cube
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